Begin with a train ride from Rome Termini to Naples, then transfer to the Circumvesuviana line for Sorrento. Booking tickets individually through Trenitalia or Italo is recommended for this route, as regional trains are not covered by the Eurail Pass and buying individual tickets ensures best prices.
Take a scenic SITA bus or boat tour to Amalfi and Positano. Since these buses are local and boats are private operators, tickets should be purchased individually. Eurail Pass does not cover these.
Travel back to Naples and take a high-speed train to Florence; buying individual high-speed tickets in advance is cost-effective, as Eurail Pass prices might exceed point-to-point tickets if not utilized fully.
Explore La Spezia, then use local trains between Cinque Terre villages. For these regional trains, purchase the Cinque Terre Card which includes train use and trail access—this is more cost-effective than using a Eurail Pass here.
Hike the scenic coastal trail between villages using the Cinque Terre Card for access. This card offers better value than a Eurail Pass on regional routes here.
For this long-distance high-speed route, it is recommended to buy advance single tickets instead of Eurail Pass to save money, unless many more long-distance trips are planned to justify the pass.